Document Details

Document Type : Article In Journal 
Document Title :
Kumosani T. FT-IR Spectroscopy as a Tool for Identification of Apoptosis-Induced Structural Changes in A549 Cells Dry Samples Treated with PM 701
Kumosani تي فاينانشيال تايمز ، الأشعة تحت الحمراء الطيفي كأداة لتحديد التغيرات الهيكلية أبوبتوسس - A549 المستحثة في عينات الخلايا المعالجة الجافة مع رئيس الوزراء 701
 
Subject : Kumosani T. FT-IR Spectroscopy as a Tool for Identification of Apoptosis-Induced Structural Changes in A549 Cells Dry Samples Treated with PM 701 
Document Language : English 
Abstract : Fourier transform infrared (FTIR) spectroscopy has been found useful for detecting apoptotic changes induced in human lung cancer cells (A549) that treated with PM 701 (natural product) in tissue culture level. Characteristic bands alterations were identified in the apoptotic cells arising from cellular protein, lipid and DNA, there were specific changes that affected the secondary structure of proteins in the apoptotic lung cells appeared in the FTIR spectra confirmed with the second derivative analysis. To follow the induced changes in the apoptotic A549 cells, different band absorbance ratios were calculated for different times up to 24 hours. The results show that there is a marked decrease in the phosphate absorbance ratio from nucleic acids, the minimum value was observed at five minutes of PM 701 treatment meanwhile there is a sharp increase in the lipid-protein ratio the maximum value was obtained for cells treated at five minutes with PM 701. This study suggests that FTIR spectroscopy could possibly be used to monitor apoptosis in A549 cells and prove the efficiency of our new drug PM 701 in cancer treatment. 
ISSN : 1319-1004 
Journal Name : Medical Science Journal 
Volume : 2 
Issue Number : 1 
Publishing Year : 1429 AH
2009 AD 
Article Type : Article 
Added Date : Saturday, March 5, 2011 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
فاتن خورشيدkhorshed, fathen ResearcherDoctorate 

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